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  • br D Daddiouaissa et al br the therapeutic

    2020-08-02


    D. Daddiouaissa, et al.
    the therapeutic effects of the fruit extract as well as the determination of the active compounds responsible for cytotoxicity.
    Conflicts of interest
    There is no conflict of interest in this work.
    Acknowledgment
    The authors are grateful to the International Islamic University Malaysia, for providing the P-RIGS grant and laboratory facilities to carry out the research.
    Appendix K 252a A. Supplementary data
    References
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    from some selected communities in Ghana by instrumental neutron activation ana-lysis. Elixir Food Sci 41, 5671–5675. Halliwell, B., 2012. Free radicals and antioxidants: updating a personal view. Nutr. Rev.
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    Contents lists available at ScienceDirect
    Life Sciences
    journal homepage: www.elsevier.com/locate/lifescie
    Antiproliferative and apoptotic activities of 8-prenylnaringenin against T
    human colon cancer cells
    Sanaz Koosha, Zahurin Mohamed, Ajantha Sinniah, Zaridatul Aini Ibrahim, Atefehalsadat Seyedan, Mohammed A. Alshawsh
    Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
    Keywords:
    Colon cancer
    8-Prenylnaringenin
    Apoptosis
    Cell cycle arrest
    Caspases
    Anti-proliferation 
    Aims: The compound 8-prenylnaringenin (8-PN) is a prenylflavonoid that can be isolated from hops and beer and has anti-cancer properties against breast cancer. The aim of this study is to investigate the anti-proliferative and apoptotic activities of 8-PN against human colon cancer HCT-116 cells.
    Main methods: Colon cancer HCT-116 cells were treated with 8-PN and subjected to MTT and acridine orange/ propidium iodide (AO/PI) staining to investigate the cytotoxicity of 8-PN. Arrest of the cells at different phases of cell cycle was monitored in the presence of 8-PN. Moreover, the apoptotic effects of 8-PN was assessed via annexin V and caspase activity assays and compared to the untreated cells. Key findings: The findings showed that 8-PN revealed strong inhibitory effect against HCT-116 cells with an IC50 value of 23.83 ± 2.9 μg/ml after 48 h. However, at similar concentrations and experimental time-points, the compound did not show cytotoxic effect to non-cancerous colon cells (CCD-41). Annexin-V assay indicates that 38.5% and 14.4% of HCT-116 cells had entered early and late stages of apoptosis, respectively after exposure of the cells to 8-PN for 48 h. Caspase activity assay illustrates that apoptosis is activated through both intrinsic and extrinsic pathways. Moreover, flow cytometry cell cycle results indicate that treatment with 8-PN significantly arrested the HCT-116 cells at G0/G1 phase.
    Significance: These findings reveal that 8-PN has anti-proliferative activity against HCT-116 colon cancer cells via induction of intrinsic and extrinsic pathway-mediated apoptosis. Further investigations should be carried out to unravel the mechanistic pathways underlying these activities.